More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| fibroblast growth factor 1(acidic) | Polyclonal | IgG | Rabbit | Human | ELISA, Neu, IP, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human FGF1 recombinant protein(Position: F16-D155). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FGF1 |
Protein | Fibroblast growth factor 1 |
Uniprot ID | P05230 |
Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. |
Tissue Specificity | Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. |
Sub-cellular localization | Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Note: Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. |
Sequence Similarities | Belongs to the heparin-binding growth factors family. |
Aliases | Acidic fibroblast growth factor antibody|AFGF antibody|Beta endothelial cell growth factor antibody|Beta-endothelial cell growth factor antibody|ECGF antibody|ECGF beta antibody|ECGF-beta antibody|ECGFA antibody|ECGFB antibody|Endothelial cell growth factor alpha antibody|Endothelial Cell Growth Factor beta antibody|FGF 1 antibody|FGF alpha antibody|Fgf1 antibody|FGFA antibody|Fibroblast Growth Factor 1 Acidic antibody|Fibroblast growth factor 1 antibody|GLIO703 antibody|HBGF 1 antibody|HBGF-1 antibody|HBGF1 antibody|Heparin binding growth factor 1 antibody|Heparin binding growth factor 1 precursor antibody|Heparin-binding growth factor 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
| Neutralization | Human | Human | |
| Immunoprecipitation | Human | Human |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-human FGF1 antibody, ASA-B0937, Western blotting
Lane 1: Recombinant Human FGF1 Protein 10ng
Lane 2: Recombinant Human FGF1 Protein 5ng
Lane 3: Recombinant Human FGF1 Protein 2
Lane 1: Recombinant Human FGF1 Protein 10ng
Lane 2: Recombinant Human FGF1 Protein 5ng
Lane 3: Recombinant Human FGF1 Protein 2
Anti-human FGF1 antibody, ASA-B0937, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
