human EPO Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
erythropoietin	Polyclonal	IgG	Rabbit	Human	ELISA, IP, IHC-P, WB	Immunogen affinity purified.
Immunogen
E. coli-derived human EPO recombinant protein(Position: A1-R166).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	EPO
Protein	Erythropoietin
Uniprot ID	P01588
Function	Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Tissue Specificity	Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Sub-cellular localization	Secreted.
Sequence Similarities	Belongs to the EPO/TPO family.
Aliases	EP antibody|EPO alpha antibody|EPO antibody|Epoetin antibody|Erythropoetin antibody|Erythropoietin antibody|Erythropoietin precursor antibody|MGC138142 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit
ELISA	0.1-0.5μg/ml	Human	Sandwich ELISA format
Immunoprecipitation	Human	Human

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information