More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
carcinoembryonic antigen-related cell adhesion molecule 5 | Polyclonal | IgG | Rabbit | Human | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen |
Insect cells-derived human CEA recombinant protein(Position: full). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CEACAM5 |
Protein | Carcinoembryonic antigen-related cell adhesion molecule 5 |
Uniprot ID | P06731 |
Function | Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. |
Tissue Specificity | Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the immunoglobulin superfamily. CEA family. |
Aliases | Carcinoembryonic antigen antibody|Carcinoembryonic antigen related cell adhesion molecule 5 antibody|Carcinoembryonic antigen-related cell adhesion molecule 5 antibody|CD66e antibody|CD66e antigen antibody|CEA antibody|Ceacam5 antibody|Meconium antigen 100 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-human CEACAM5 antibody, ASA-B0934, Western blotting
Lane 1: Recombinant Human CEA Protein 5ng
Lane 2: Recombinant Human CEA Protein 2