More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 27kDa protein 2(HspB2) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human HSPB2(102-118aa RHPQRLDRHGFVSREFC), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPB2 |
Protein | Heat shock protein beta-2(HspB2) |
Uniprot ID | Q16082 |
Function | May regulate the kinase DMPK. |
Tissue Specificity | Expressed preferentially in skeletal muscle and heart but not in the lens. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the small heat shock protein (HSP20) family. |
Aliases | DMPK-binding protein antibody|Heat shock 27kD protein 2 antibody|Heat shock 27kDa protein 2 antibody|Heat shock protein beta-2 antibody|Heat-shock protein beta-2 antibody|Hs.78846 antibody|HSP27 antibody|HspB2 antibody|HSPB2_HUMAN antibody|LOH11CR1K antibody|MGC133245 antibody|MKBP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSPB2 antibody, ASA-B0928, ICC
ICC: HELA Cell
ICC: HELA Cell
Anti-HSPB2 antibody, ASA-B0928, Western blotting
All lanes: Anti HSPB2 (ASA-B0928) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 20KD
Observed bind size: 20KD
All lanes: Anti HSPB2 (ASA-B0928) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 20KD
Observed bind size: 20KD
Anti-HSPB2 antibody, ASA-B0928, IHC(F)
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Rat Cardiac Muscle Tissue