Hsp90 alpha Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
heat shock protein 90kDa alpha (cytosolic), class A member 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, ICC, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human Hsp90 recombinant protein (Position: P2-V365). Human Hsp90 shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HSP90AA1
Protein	Heat shock protein HSP 90-alpha
Uniprot ID	P07900
Function	Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Tissue Specificity
Sub-cellular localization	Cytoplasm. Melanosome. Cell membrane. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Sequence Similarities
Aliases	EL52 antibody|epididymis luminal secretory protein 52 antibody|Heat shock 86 kDa antibody|heat shock 90kD protein 1, alpha antibody| Heat shock 90kD protein 1, alpha like 4 antibody|heat shock 90kD protein, alpha-like 4 antibody|Heat shock 90kDa protein 1 alpha antibody|Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody|heat shock protein 90kDa alpha (cytosolic), class A member 2 antibody|Heat shock protein HSP 90-alpha antibody|HS90A_HUMAN antibody|HSP 86 antibody|HSP 86 antibody|HSP86 antibody|Hsp89 antibody|HSP89A antibody|Hsp90 antibody|HSP90A antibody|HSP90AA1 antibody|HSP90ALPHA antibody|HSP90N antibody|HSPC1 antibody|HSPCA antibody|HSPCAL1 antibody|HSPCAL3 antibody|HSPCAL4 antibody|HSPN antibody|LAP 2 antibody|LAP2 antibody|lipopolysaccharide-associated protein 2 antibody|LPS-associated protein 2 antibody|Renal carcinoma antigen NY REN 38 antibody|Renal carcinoma antigen NY-REN-38 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information