More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 70kDa protein 1A; heat shock 70kDa protein 1B | Monoclonal | IgG | Mouse | Bovine, Human, Rabbit, Rat | IHC-P, IHC-F, WB | Ascites |
Immunogen |
| HSP70 isolated from bovine brain. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPA1A; HSPA1B |
Protein | Heat shock 70 kDa protein 1A/1B |
Uniprot ID | Human(P08107), Mouse(Q61696), Rat(Q07439), Mouse(P17879) |
Function | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223). |
Tissue Specificity | HSPA1B is testis-specific. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the heat shock protein 70 family. |
Aliases | HSPA1|Hsp70-1|Hsp70-2|Heat shock 70 kDa protein 1/2 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5μg/ml | Human, bovine, rabbit, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, bovine, rabbit, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, bovine, rabbit, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
