More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 60kDa protein 1 (chaperonin) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPD1 |
Protein | 60 kDa heat shock protein, mitochondrial |
Uniprot ID | P10809 |
Function | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion matrix. |
Sequence Similarities | Belongs to the chaperonin (HSP60) family. |
Aliases | 60 kDa chaperonin antibody|60 kDa heat shock protein, mitochondrial antibody|60kDa antibody|cb863 antibody|CH60_HUMAN antibody|Chaperonin 60 antibody|Chaperonin antibody|Chaperonin, 60-KD antibody|CPN60 antibody|GROEL antibody|GroEL, E. coli, homolog of antibody|Heat shock 60kD protein 1 (chaperonin) antibody|heat shock 60kDa protein 1 (chaperonin) antibody|Heat shock 60kDa protein 1 antibody|Heat shock protein 1 (chaperonin) antibody|Heat shock protein 60 antibody|Heat shock protein 65 antibody|HLD4 antibody|Hsp 60 antibody|HSP 65 antibody|HSP-60 antibody|HSP60 antibody|HSP65 antibody|HSPD1 antibody|HuCHA60 antibody|Mitochondrial matrix protein P1 antibody|P60 lymphocyte protein antibody|Short heat shock protein 60 Hsp60s1 antibody|Spastic paraplegia 13 (autosomal dominant) antibody|SPG13 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Hsp60 antibody, ASA-B0917, Western blotting
All lanes: Anti Hsp60 (ASA-B0917) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Lane 4: NRK Whole Cell Lysate at 40ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: A431 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40
All lanes: Anti Hsp60 (ASA-B0917) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Mouse Kidney Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Lane 4: NRK Whole Cell Lysate at 40ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: A431 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40
Anti- Hsp60 antibody, ASA-B0917, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- Hsp60 antibody, ASA-B0917, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue