Hsp40 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
DnaJ(Hsp40) homolog, subfamily B, member 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp40(317-332aa EFEVIFPERIPQTSRT), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	DNAJB1
Protein	DnaJ homolog subfamily B member 1
Uniprot ID	P25685
Function	Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Tissue Specificity
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Contains 1 J domain.
Aliases	DnaJ(Hsp40) homolog subfmaily B member 1 antibody|DNAJ 1 antibody|DNAJ B1 antibody|DnaJ homolog subfamily B member 1 antibody|DnaJ protein homolog 1 antibody|DNAJ1 antibody|DNAJB 1 antibody|DNAJB1 antibody|DNAJB1 protein antibody|DNJB1_HUMAN antibody|HDJ 1 antibody|HDJ-1 antibody|HDJ1 antibody|Heat shock 40 kDa protein 1 antibody|Heat shock 40kD protein 1 antibody|Heat shock protein 40 antibody|Hsp 40 antibody|Hsp40 antibody|HSPF 1 antibody|HSPF1 antibody|Human DnaJ protein 1 antibody|Radial spoke 16 homolog B antibody|RSPH16B antibody|Sis1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information