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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 27kDa protein 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPB1 |
Protein | Heat shock protein beta-1 |
Uniprot ID | P04792 |
Function | Involved in stress resistance and actin organization. |
Tissue Specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
Sub-cellular localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
Sequence Similarities | Belongs to the small heat shock protein (HSP20) family. |
Aliases | Heat shock 27kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25kDa protein 1 antibody|Heat shock 25kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27kDa protein 1 antibody|Heat shock 27kDa protein 1 antibody|Heat shock 28kDa protein 1 antibody|Heat shock 28kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|Heat Shock Protein27 antibody|Heat Shock Protein27 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|Hsp 25 antibody|Hsp 27 antibody|Hsp 27 antibody|Hsp 28 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp B1 antibody|Hsp25 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|Hsp28 antibody|HspB1 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HSP27 antibody, ASA-B0912, Western blotting
All lanes: Anti HSP27 (ASA-B0912) at 0.5ug/ml
WB: Recombinant Human HSP27 Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
All lanes: Anti HSP27 (ASA-B0912) at 0.5ug/ml
WB: Recombinant Human HSP27 Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
Anti- HSP27 antibody, ASA-B0912, Western blotting
All lanes: Anti HSP27 (ASA-B0912) at 0.5ug/ml
Lane 1: NRK Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti HSP27 (ASA-B0912) at 0.5ug/ml
Lane 1: NRK Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Anti- HSP27 antibody, ASA-B0912, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue