More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 27kDa protein 1 | Monoclonal | IgG | Mouse | Human, Mouse, Rabbit, Rat | IHC-P, IHC-F, ICC, WB | Ascites |
Immunogen |
| Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPB1 |
Protein | Heat shock protein beta-1 |
Uniprot ID | Human(P04792), Mouse(P14602), Rat(P42930) |
Function | Involved in stress resistance and actin organization. |
Tissue Specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
Sub-cellular localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
Sequence Similarities | Belongs to the small heat shock protein (HSP20) family. |
Aliases | HSP27|HSP28|CMT2F|HMN2B|28 kDa heat shock protein|Estrogen-regulated 24 kDa protein|Heat shock 27 kDa protein |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-2μg/ml | Human, mouse, rabbit, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, mouse, rabbit, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 1-2μg/ml | Human, mouse, rabbit, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human, mouse, rabbit, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
