Hsp105 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
heat shock 105kDa/110kDa protein 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HSPH1
Protein	Heat shock protein 105 kDa
Uniprot ID	Q92598
Function	Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).
Tissue Specificity	Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Belongs to the heat shock protein 70 family.
Aliases	Antigen NY CO 25 antibody|Antigen NY-CO-25 antibody|DKFZp686M05240 antibody|Heat shock 105kD alpha antibody|Heat shock 105kD antibody|Heat shock 105kD beta antibody|Heat shock 105kDa protein 1 antibody|Heat shock 105kDa protein antibody|Heat shock 105kDa/110kDa protein 1 antibody|Heat shock 110 kDa protein antibody|Heat shock 110kDa protein antibody|Heat shock protein 105 kDa antibody|HS105_HUMAN antibody|HSP105 antibody|HSP105A antibody|HSP105B antibody|HSP110 antibody|HSPH 1 antibody|HSPH1 antibody|KIAA0201 antibody|NY CO 25 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Rat Human, Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information