More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 105kDa/110kDa protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPH1 |
Protein | Heat shock protein 105 kDa |
Uniprot ID | Q92598 |
Function | Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity). |
Tissue Specificity | Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the heat shock protein 70 family. |
Aliases | Antigen NY CO 25 antibody|Antigen NY-CO-25 antibody|DKFZp686M05240 antibody|Heat shock 105kD alpha antibody|Heat shock 105kD antibody|Heat shock 105kD beta antibody|Heat shock 105kDa protein 1 antibody|Heat shock 105kDa protein antibody|Heat shock 105kDa/110kDa protein 1 antibody|Heat shock 110 kDa protein antibody|Heat shock 110kDa protein antibody|Heat shock protein 105 kDa antibody|HS105_HUMAN antibody|HSP105 antibody|HSP105A antibody|HSP105B antibody|HSP110 antibody|HSPH 1 antibody|HSPH1 antibody|KIAA0201 antibody|NY CO 25 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Hsp105 antibody, ASA-B0909, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: Rat Ovary Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Anti-Hsp105 antibody, ASA-B0909, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Hsp105 antibody, ASA-B0909, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue