More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hydroxysteroid(17-beta) dehydrogenase 6 homolog(mouse) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6(300-317aa SLADYILTRSWPKPAQAV). | ||||||
Properties
Form | Liquid |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request. |
Concentration | |
Storage | At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSD17B6 |
Protein | 17-beta-hydroxysteroid dehydrogenase type 6(17-beta-HSD 6/17-beta-HSD6) |
Uniprot ID | O14756 |
Function | NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3- alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha- androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids. |
Tissue Specificity | Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain. |
Sub-cellular localization | Microsome membrane. |
Sequence Similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
Aliases | 17-beta-HSD 6 antibody|17-beta-HSD6 antibody|17-beta-hydroxysteroid dehydrogenase type 6 antibody|3 hydroxysteroid epimerase antibody|3(alpha>beta) hydroxysteroid epimerase antibody|3(alpha>beta) hydroxysteroid epimerasel antibody|3-alpha->beta-HSE antibody|3-alpha->beta-hydroxysteroid epimerase antibody|H17B6_HUMAN antibody|HSD17B6 antibody|HSE antibody|Hydroxysteroid(17 beta) dehydrogenase 6 antibody|Hydroxysteroid(17 beta) dehydrogenase 6 homolog(mouse) antibody|Hydroxysteroid 17 beta dehydrogenase 6 antibody|NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase antibody|NAD+ dependent 3 alpha hydroxysteroid dehydrogenase antibody|Oxidative 3 alpha hydroxysteroid dehydrogenase antibody|Oxidative 3-alpha hydroxysteroid dehydrogenase antibody|Oxidoreductase antibody|Retinol dehydrogenase antibody|RODH antibody|SDR9C6 antibody|Short chain dehydrogenase/reductase family 9C, member 6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HSD17B6 antibody, ASA-B0905, Western blotting
All lanes: Anti HSD17B6 (ASA-B0905) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti HSD17B6 (ASA-B0905) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
