HSD17B6 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
hydroxysteroid(17-beta) dehydrogenase 6 homolog(mouse)	Polyclonal	IgG	Rabbit	Human	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6(300-317aa SLADYILTRSWPKPAQAV).

Properties

Form	Liquid
Size	100 µg/vial
Contents	Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request.
Concentration
Storage	At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HSD17B6
Protein	17-beta-hydroxysteroid dehydrogenase type 6(17-beta-HSD 6/17-beta-HSD6)
Uniprot ID	O14756
Function	NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3- alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha- androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
Tissue Specificity	Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
Sub-cellular localization	Microsome membrane.
Sequence Similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Aliases	17-beta-HSD 6 antibody|17-beta-HSD6 antibody|17-beta-hydroxysteroid dehydrogenase type 6 antibody|3 hydroxysteroid epimerase antibody|3(alpha>beta) hydroxysteroid epimerase antibody|3(alpha>beta) hydroxysteroid epimerasel antibody|3-alpha->beta-HSE antibody|3-alpha->beta-hydroxysteroid epimerase antibody|H17B6_HUMAN antibody|HSD17B6 antibody|HSE antibody|Hydroxysteroid(17 beta) dehydrogenase 6 antibody|Hydroxysteroid(17 beta) dehydrogenase 6 homolog(mouse) antibody|Hydroxysteroid 17 beta dehydrogenase 6 antibody|NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase antibody|NAD+ dependent 3 alpha hydroxysteroid dehydrogenase antibody|Oxidative 3 alpha hydroxysteroid dehydrogenase antibody|Oxidative 3-alpha hydroxysteroid dehydrogenase antibody|Oxidoreductase antibody|Retinol dehydrogenase antibody|RODH antibody|SDR9C6 antibody|Short chain dehydrogenase/reductase family 9C, member 6 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information