More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hydroxysteroid(17-beta) dehydrogenase 2 | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2(373-387aa MPRALRMPNYKKKAT). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSD17B2 |
Protein | Estradiol 17-beta-dehydrogenase 2 |
Uniprot ID | P37059 |
Function | Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH. |
Tissue Specificity | |
Sub-cellular localization | Membrane ; Single-pass type II membrane protein . |
Sequence Similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
Aliases | 17 beta HSD 2 antibody|17-beta-HSD 2 antibody|17-beta-hydroxysteroid dehydrogenase type 2 antibody|20 alpha HSD antibody|20 alpha hydroxysteroid dehydrogenase antibody|20 alpha-hydroxysteroid dehydrogenase antibody|20-alpha-HSD antibody|DHB2_HUMAN antibody|E2DH antibody|EDH17B2 antibody|Estradiol 17-beta-dehydrogenase 2 antibody|HSD17 antibody|Hsd17b2 antibody|Hydroxysteroid(17 beta) dehydrogenase 2 antibody|Microsomal 17 beta hydroxysteroid dehydrogenase antibody|Microsomal 17-beta-hydroxysteroid dehydrogenase antibody|SDR9C2 antibody|Testosterone 17 beta dehydrogenase antibody|Testosterone 17-beta-dehydrogenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSD17B2 antibody, ASA-B0904, Western blotting
All lanes: Anti HSD17B2 (ASA-B0904) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD
All lanes: Anti HSD17B2 (ASA-B0904) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD
Anti-HSD17B2 antibody, ASA-B0904, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-HSD17B2 antibody, ASA-B0904, IHC(F)
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue