More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hydroxysteroid(17-beta) dehydrogenase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1(29-43aa QSFKVYATLRDLKTQ), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSD17B1 |
Protein | Estradiol 17-beta-dehydrogenase 1 |
Uniprot ID | P14061 |
Function | Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
Aliases | 17 beta HSD 1 antibody|17 beta hydroxysteroid dehydrogenase type 1 antibody|17-beta-HSD 1 antibody|17-beta-hydroxysteroid dehydrogenase type 1 antibody|20 alpha-hydroxysteroid dehydrogenase antibody|20-alpha-HSD antibody|DHB1_HUMAN antibody|E17KSR antibody|E2DH antibody|EDH17B1 antibody|EDH17B2 antibody|EDHB17 antibody|Estradiol 17 beta dehydrogenase 1 antibody|Estradiol 17-beta-dehydrogenase 1 antibody|HSD17 antibody|HSD17B1 antibody|Hydroxysteroid(17 beta) dehydrogenase 1 antibody|MGC13814 antibody|Placental 17 beta hydroxysteroid dehydrogenase antibody|Placental 17-beta-hydroxysteroid dehydrogenase antibody|SDR28C1 antibody|Short chain dehydrogenase/reductase family 28CE,member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSD17B1 antibody, ASA-B0901, ICC
ICC: HELA Cell
ICC: HELA Cell
Anti-HSD17B1 antibody, ASA-B0901, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: HELA Cell Lysate
Anti-HSD17B1 antibody, ASA-B0901, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue