HSD11B1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
hydroxysteroid(11-beta) dehydrogenase 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HSD11B1(131-145aa DDIHHVRKSMEVNFL), different from the mouse sequence by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HSD11B1
Protein	Corticosteroid 11-beta-dehydrogenase isozyme 1
Uniprot ID	P28845
Function	Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol (By similarity).
Tissue Specificity	Widely expressed. Highest expression in liver.
Sub-cellular localization	Endoplasmic reticulum membrane.
Sequence Similarities
Aliases	11 beta HSD 1 antibody|11 beta HSD1 antibody|11 beta hydroxysteroid dehydrogenase 1 antibody|11 DH antibody|11-beta hydroxysteroid dehydrogenase, type 1 antibody|11-beta-HSD1 antibody|11-beta-hydroxysteroid dehydrogenase 1 antibody|11-DH antibody|11DH antibody|Corticosteroid 11 beta dehydrogenase isozyme 1 antibody|Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody|CORTRD2 antibody|DHI1_HUMAN antibody|HDL antibody|HSD 11 antibody|HSD11 antibody|HSD11B antibody|HSD11B1 antibody|HSD11L antibody|Hydroxysteroid(11 beta) dehydrogenase 1 antibody|Hydroxysteroid(11 beta) dehydrogenase antibody|MGC13539 antibody|SDR26C1 antibody|short chain dehydrogenase/reductase family 26C, member 1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Mouse, Rat	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information