More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hydroxysteroid(11-beta) dehydrogenase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1(16-33aa MAYYYYSANEEFRPEMLQ), different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSD11B1 |
Protein | Corticosteroid 11-beta-dehydrogenase isozyme 1 |
Uniprot ID | P28845 |
Function | Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol (By similarity). |
Tissue Specificity | Widely expressed. Highest expression in liver. |
Sub-cellular localization | Endoplasmic reticulum membrane. |
Sequence Similarities | |
Aliases | 11 beta HSD 1 antibody|11 beta HSD1 antibody|11 beta hydroxysteroid dehydrogenase 1 antibody|11 DH antibody|11-beta hydroxysteroid dehydrogenase, type 1 antibody|11-beta-HSD1 antibody|11-beta-hydroxysteroid dehydrogenase 1 antibody|11-DH antibody|11DH antibody|Corticosteroid 11 beta dehydrogenase isozyme 1 antibody|Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody|CORTRD2 antibody|DHI1_HUMAN antibody|HDL antibody|HSD 11 antibody|HSD11 antibody|HSD11B antibody|HSD11B1 antibody|HSD11L antibody|Hydroxysteroid(11 beta) dehydrogenase 1 antibody|Hydroxysteroid(11 beta) dehydrogenase antibody|MGC13539 antibody|SDR26C1 antibody|short chain dehydrogenase/reductase family 26C, member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSD11B1 (ASA-B0899) antibody, Western blotting
All lanes: All lanes: Anti HSD11B1 (ASA-B0899) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: RAJI Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD
All lanes: All lanes: Anti HSD11B1 (ASA-B0899) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: RAJI Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD
Anti-HSD11B1 (ASA-B0899) antibody, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
