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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock 70kDa protein 8 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Hsc70(13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSPA8 |
Protein | Heat shock cognate 71 kDa protein |
Uniprot ID | E9PSH5 |
Function | |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | |
Aliases | 2410008N15Rik antibody|Constitutive heat shock protein 70 antibody|Heat shock 70 kDa protein 8 antibody|Heat shock 70kD protein 10 antibody|Heat shock 70kD protein 8 antibody|Heat shock 70kDa protein 8 antibody|Heat shock cognate 71 kDa protein antibody|Heat shock cognate 71 kDa protein. antibody|Heat shock cognate protein 54 antibody|Heat shock cognate protein 71 kDa antibody|Heat shock cognate protein 71-kDa antibody|Heat shock protein 8 antibody|Heat shock protein A8 antibody|Heat-shock70-KD protein 10, formerly antibody|HSC54 antibody|HSC71 antibody|Hsc73 antibody|HSP71 antibody|HSP73 antibody|HSP7C_HUMAN antibody|HSPA10 antibody|HSPA8 antibody|LAP1 antibody|Lipopolysaccharide associated protein 1 antibody|LPS associated protein 1 antibody|LPS associated protein antibody|MGC102007 antibody|MGC106514 antibody|MGC114311 antibody|MGC118485 antibody|MGC131511 antibody|MGC29929 antibody|N-myristoyltransferase inhibitor protein 71 antibody|NIP71 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Hsc70 antibody, ASA-B0895, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Lane 5: CEM Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: COLO320 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Lane 5: CEM Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: COLO320 Cell Lysate
Anti-Hsc70 antibody, ASA-B0895, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Hsc70 antibody, ASA-B0895, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue