HOXA4 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
homeobox A4	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HOXA4(9-26aa NSNYIEPKFPPFEEYAQH), different from the related mouse sequence by two amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HOXA4
Protein	Homeobox protein Hox-A4
Uniprot ID	Q00056
Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT- 3'.
Tissue Specificity	Embryonic nervous system.
Sub-cellular localization	Nucleus.
Sequence Similarities	Belongs to the Antp homeobox family. Deformed subfamily.
Aliases	Dfd like protein antibody|Homeo box A4 antibody|Homeobox A4 antibody|Homeobox protein Hox-1.4 antibody|Homeobox protein Hox-1D antibody|Homeobox protein Hox-A4 antibody|Hox 1.4 like protein antibody|hox-1.4 antibody|hox-1d antibody|HOX1 antibody|HOX1D antibody|HOXA4 antibody|HXA4_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human Mouse	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information