More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
homeobox A3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human HOXA3(427-443aa HHPSQGRIQEAPKLTHL), identical to the related rat and mouse sequences. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HOXA3 |
Protein | Homeobox protein Hox-A3 |
Uniprot ID | O43365 |
Function | Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the Antp homeobox family. |
Aliases | Homeo box 1E antibody|Homeo box A3 antibody|Homeobox 1E antibody|Homeobox A3 antibody|Homeobox protein HOX A3 antibody|Homeobox protein Hox-1E antibody|Homeobox protein Hox-A3 antibody|Homeobox protein HOXA3 antibody|Homeobox1E antibody|HomeoboxA3 antibody|HOX 1 antibody|Hox 1.5 like protein antibody|HOX 1E antibody|HOX A3 antibody|HOX1 antibody|Hox1.5 antibody|Hox1.5 like protein antibody|HOX1E antibody|HOXA 3 antibody|HOXA3 antibody|HXA3_HUMAN antibody|MGC10155 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HOXA3 antibody, ASA-B0888, Western blotting
All lanes: Anti HOXA3 (ASA-B0888) at 0.5ug/ml
WB: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 46KD
Observed bind size: 46KD
Anti-HOXA3 antibody, ASA-B0888, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue