HNF6 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
one cut homeobox 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HNF6(187-203aa LGSIHNSQQGLPHYAHP), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	ONECUT1
Protein	Hepatocyte nuclear factor 6
Uniprot ID	Q9UBC0
Function	Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Tissue Specificity	Highly expressed in liver; lower expression in testis and skin.
Sub-cellular localization	Nucleus.
Sequence Similarities	Belongs to the CUT homeobox family.
Aliases	Hepatocyte nuclear factor 6 antibody|HNF 6 antibody|HNF6A antibody|One cut domain family member 1 antibody|Onecut1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Rat Human, Mouse	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information