More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hepatocyte nuclear factor 4, alpha | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HNF4A |
Protein | Hepatocyte nuclear factor 4-alpha |
Uniprot ID | P41235 |
Function | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR2 subfamily. |
Aliases | FLJ39654 antibody|Hepatic nuclear factor 4 alpha antibody|Hepatocyte nuclear factor 4 alpha antibody|Hepatocyte nuclear factor 4 antibody|Hepatocyte nuclear factor 4-alpha antibody|HNF 4 alpha antibody|HNF 4 antibody|HNF-4-alpha antibody|HNF4 antibody|HNF4 antibody|HNF4A antibody|HNF4A_HUMAN antibody|HNF4a7 antibody|HNF4a8 antibody|HNF4a9 antibody|Hnf4alpha antibody|HNF4alpha10/11/12 antibody|MODY 1 antibody|MODY antibody|MODY1 antibody|NR2A1 antibody|NR2A1 antibody|NR2A21 antibody|Nuclear receptor subfamily 2 group A member 1 antibody|OTTHUMP00000031060 antibody|OTTHUMP00000031062 antibody|TCF 14 antibody|TCF antibody|TCF-14 antibody|TCF14 antibody|TCF14 antibody|Tcf4 antibody|Transcription factor 14, hepatic nuclear factor antibody|Transcription factor 14 antibody|Transcription factor HNF 4 antibody|Transcription factor HNF-4 antibody|Transcription factor HNF4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HNF-4-alpha antibody, ASA-B0879, Western blotting
All lanes: Anti HNF-4-alpha (ASA-B0879) at 0.5ug/ml
WB: Recombinant Human HNF-4-alpha Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti HNF-4-alpha (ASA-B0879) at 0.5ug/ml
WB: Recombinant Human HNF-4-alpha Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- HNF-4-alpha antibody, ASA-B0879, Western blotting
All lanes: Anti HNF-4-alpha (ASA-B0879) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
All lanes: Anti HNF-4-alpha (ASA-B0879) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
Anti- HNF-4-alpha antibody, ASA-B0879, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue