HNF1 beta Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
HNF1 homeobox B	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HNF1 beta(494-509aa HMAQQPFMAAVTQLQN), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	HNF1B
Protein	Hepatocyte nuclear factor 1-beta
Uniprot ID	P35680
Function	Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Tissue Specificity
Sub-cellular localization	Nucleus.
Sequence Similarities	Belongs to the HNF1 homeobox family.
Aliases	FJHN antibody|Hepatocyte nuclear factor 1 beta antibody|Hepatocyte nuclear factor 1-beta antibody|HNF 1B antibody|HNF 2 antibody|HNF-1-beta antibody|HNF-1B antibody|HNF1 beta antibody|HNF1 homeobox B antibody|HNF1B antibody|HNF1beta antibody|HNF2 antibody|Homeoprotein LF B3 antibody|Homeoprotein LFB3 antibody|HPC11 antibody|LF B3 antibody|LFB3 antibody|MODY 5 antibody|MODY5 antibody|TCF 2 antibody|TCF 2 protein antibody|TCF-2 antibody|TCF2 antibody|TCF2 protein antibody|Transcription factor 2 antibody|Transcription factor 2 hepatic antibody|Variant hepatic nuclear factor 1 antibody|Variant hepatic nuclear factor antibody|VHNF 1 antibody|vHNF1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse Human, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Mouse Human, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information