More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heme oxygenase (decycling) 2 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human HMOX2 recombinant protein (Position: S2-M316). Human HMOX2 shares 89% and 90% amino acid (aa) sequences identity with mouse and rat HMOX2, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HMOX2 |
Protein | Heme oxygenase 2 |
Uniprot ID | P30519 |
Function | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. |
Tissue Specificity | |
Sub-cellular localization | Microsome. Endoplasmic reticulum. |
Sequence Similarities | Belongs to the heme oxygenase family. |
Aliases | eme oxygenase (decycling) 2 antibody|Heme oxygenase (decyclizing) 2 antibody|Heme oxygenase 2 antibody|HMOX 2 antibody|HMOX2 antibody|HMOX2 protein antibody|HMOX2_HUMAN antibody|HO 2 antibody|HO-2 antibody|HO2 antibody|OTTHUMP00000159847 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HMOX2 antibody, ASA-B0878, Western blotting
All lanes: Anti HMOX2 (ASA-B0878) at 0.5ug/ml
WB: Recombinant Human HMOX2 Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti HMOX2 (ASA-B0878) at 0.5ug/ml
WB: Recombinant Human HMOX2 Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti- HMOX2 antibody, ASA-B0878, Western blotting
All lanes: Anti HMOX2 (ASA-B0878) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti HMOX2 (ASA-B0878) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- HMOX2 antibody, ASA-B0878, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue