More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heme oxygenase (decycling) 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HMOX1 |
Protein | Heme oxygenase 1 |
Uniprot ID | P09601 |
Function | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. |
Tissue Specificity | Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). |
Sub-cellular localization | Microsome. |
Sequence Similarities | Belongs to the heme oxygenase family. |
Aliases | 32 kD antibody|bK286B10 antibody|D8Wsu38e antibody|heat shock protein 32 kD antibody|heat shock protein 32kD antibody|Heat shock protein antibody|Heme oxygenase (decycling) 1 antibody|Heme oxygenase 1 antibody|Hemox antibody|HMOX 1 antibody|Hmox antibody|HMOX1 antibody|HMOX1_HUMAN antibody|HO 1 antibody|HO antibody|HO-1 antibody|HO1 antibody|Hsp32 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HMOX1 antibody, ASA-B0877, Western blotting
All lanes: Anti HMOX1 (ASA-B0877) at 0.5ug/ml
WB: Recombinant Human HMOX1 Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti HMOX1 (ASA-B0877) at 0.5ug/ml
WB: Recombinant Human HMOX1 Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti- HMOX1 antibody, ASA-B0877, Western blotting
All lanes: Anti HMOX1 (ASA-B0877) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti HMOX1 (ASA-B0877) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Anti- HMOX1 antibody, ASA-B0877, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue