More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heme oxygenase (decycling) 1 | Polyclonal | IgG | Rabbit | Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived mouse HMOX1 recombinant protein (Position: E2-T261). Mouse HMOX1 shares 82% and 93% amino acid (aa) sequences identity with human and rat HMOX1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HMOX1 |
Protein | Heme oxygenase 1 |
Uniprot ID | P14901 |
Function | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. |
Tissue Specificity | |
Sub-cellular localization | Microsome. Endoplasmic reticulum membrane ; Peripheral membrane protein; Cytoplasmic side. |
Sequence Similarities | |
Aliases | 32 kD antibody|bK286B10 antibody|D8Wsu38e antibody|heat shock protein 32 kD antibody|heat shock protein 32kD antibody|Heat shock protein antibody|Heme oxygenase (decycling) 1 antibody|Heme oxygenase 1 antibody|Hemox antibody|HMOX 1 antibody|Hmox antibody|HMOX1 antibody|HMOX1_HUMAN antibody|HO 1 antibody|HO antibody|HO-1 antibody|HO1 antibody|Hsp32 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HMOX1 antibody, ASA-B0876--1.jpg
All lanes: Anti-HMOX1(ASA-B0876) at 0.5ug/ml
Lane 1: NIH Whole Cell Lysate at 40ug
Lane 2: NEURO Whole Cell Lysate at 40ug
Lane 3: Mouse Liver Tissue Lysate at 40ug
Lane 4: Mouse Spleen Tissue Lysate at 40ug
Lane 5: Mouse Ovary Tissue Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti-HMOX1(ASA-B0876) at 0.5ug/ml
Lane 1: NIH Whole Cell Lysate at 40ug
Lane 2: NEURO Whole Cell Lysate at 40ug
Lane 3: Mouse Liver Tissue Lysate at 40ug
Lane 4: Mouse Spleen Tissue Lysate at 40ug
Lane 5: Mouse Ovary Tissue Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
