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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| growth factor receptor-bound protein 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human GRB7(124-141aa ARHVCEMLVQRAHALSDE), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRB7 |
Protein | Growth factor receptor-bound protein 7 |
Uniprot ID | Q14451 |
Function | Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic granule . Cell projection. Note: Predominantly cytoplasmic. Detected in stress granules, where mRNA is stored under stress conditions (By similarity). |
Sequence Similarities | Belongs to the GRB7/10/14 family. |
Aliases | B47 antibody|Epidermal growth factor receptor GRB 7 antibody|Epidermal growth factor receptor GRB-7 antibody|GRB7 adapter protein antibody|GRB7 antibody|GRB7_HUMAN antibody|Growth factor receptor bound protein 7 antibody|Growth factor receptor-bound protein 7 antibody|OTTHUMP00000164349 antibody|OTTHUMP00000164350 antibody|OTTHUMP00000164351 antibody|OTTHUMP00000164352 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GRB7 antibody, ASA-B0814, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
IHC(P): Human Oesophagus Squama Cancer Tissue
Anti-GRB7 antibody, ASA-B0814, All Western blotting
All lanes: Anti-GRB7(ASA-B0814) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti-GRB7(ASA-B0814) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
