More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutaredoxin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Glutaredoxin 2(103-119aa EYGNQFQDALYKMTGER), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GLRX2 |
Protein | Glutaredoxin-2, mitochondrial |
Uniprot ID | Q9NS18 |
Function | Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. |
Tissue Specificity | Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes. |
Sub-cellular localization | Isoform 1: Mitochondrion. |
Sequence Similarities | Belongs to the glutaredoxin family. |
Aliases | bA101E13.1(GRX2 glutaredoxin(thioltransferase) 2) antibody|bA101E13.1 antibody|CGI133 antibody|Glrx2 antibody|GLRX2_HUMAN antibody|Glutaredoxin-2 antibody|Glutaredoxin-2, mitochondrial antibody|GRX2 antibody|mitochondrial antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Glutaredoxin 2 antibody, ASA-B0802, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: NEU Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: NEU Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: MCF-7 Cell Lysate
Anti-Glutaredoxin 2 antibody, ASA-B0802, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
