GLUT4 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
solute carrier family 2 (facilitated glucose transporter), member 4	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, WB	Immunogen affinity purified.
Immunogen
E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SLC2A4
Protein	Solute carrier family 2, facilitated glucose transporter member 4
Uniprot ID	P14672
Function	Insulin-regulated facilitative glucose transporter.
Tissue Specificity	Skeletal and cardiac muscles; brown and white fat.
Sub-cellular localization	Cell membrane ; Multi-pass membrane protein . Endomembrane system.
Sequence Similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Aliases	insulin-responsive antibody|Glucose transporter GLUT 4 antibody|Glucose transporter type 4 antibody|Glucose transporter type 4 insulin responsive antibody|GLUT 4 antibody|GLUT-4 antibody|GLUT4 antibody|GTR4_HUMAN antibody|kug antibody|SLC 2A4 antibody|SLC2A4 antibody|solute carrier family 2 (facilitated glucose transporter) member 4 antibody|Solute carrier family 2, facilitated glucose transporter member 4 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat Human	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Mouse, Rat Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information