More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 2(facilitated glucose transporter), member 8 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Glucose Transporter 8(461-477aa ETKGRTLEQVTAHFEGR), different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC2A8 |
Protein | Solute carrier family 2, facilitated glucose transporter member 8 |
Uniprot ID | Q9JIF3 |
Function | Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose. |
Tissue Specificity | Highest level of expression in placenta and testis. Highly expressed in adult and pubertal testis, but not prepubertal testis. Lower levels of expression in brain, liver, heart, kidney, fat and skeletal muscle. |
Sub-cellular localization | Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Note: Principally intracellular. May move between intracellular vesicles and the plasma membrane. The dileucine internalization motif is critical for intracellular sequestration (By similarity). Insulin induces a change in the intracellular localization and gives rise to insertion in the plasma membrane. |
Sequence Similarities | |
Aliases | Glucose transporter type 8 antibody|Glucose transporter type X1 antibody|GLUT8 antibody|GLUTX1 antibody|solute carrier family 2(facilitated glucose transporter) member 8 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Glucose Transporter 8 antibody, ASA-B0794, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Anti-Glucose Transporter 8 antibody, ASA-B0794, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti-Glucose Transporter 8 antibody, ASA-B0794, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue