More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| GLI family zinc finger 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human GLi1 recombinant protein (Position: Q768-A1106). Human GLi1 shares 79% amino acid (aa) sequence identity with mouse GLi1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GLI1 |
Protein | Zinc finger protein GLI1 |
Uniprot ID | P08151 |
Function | Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. |
Tissue Specificity | Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. |
Sequence Similarities | Belongs to the GLI C2H2-type zinc-finger protein family. |
Aliases | Gli 1 antibody|GLI antibody|GLI family zinc finger 1 antibody|GLI Kruppel family member 1 antibody|gli1 antibody|GLI1_HUMAN antibody|Glioma associated oncogene 1 antibody|Glioma associated oncogene antibody|Glioma associated oncogene homolog 1 (zinc finger protein) antibody|Glioma associated oncogene homolog antibody|Glioma-associated oncogene antibody|Oncogene GLI antibody|Zfp 5 antibody|Zfp5 antibody|Zinc finger protein GLI 1 antibody|Zinc finger protein GLI1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Gli1 antibody, ASA-B0788--1.jpg
All lanes: Anti GLI1 (ASA-B0788) at 0.5ug/ml
WB: Recombinant Human GLI1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti GLI1 (ASA-B0788) at 0.5ug/ml
WB: Recombinant Human GLI1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-Gli1 antibody, ASA-B0788--2.jpg
All lanes: Anti GLI1 (ASA-B0788) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 118KD
All lanes: Anti GLI1 (ASA-B0788) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 118KD
Anti-Gli1 antibody, ASA-B0788--3.jpg
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue