More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| gelsolin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Gelsolin recombinant protein (Position: E580-A782). Human Gelsolin shares 94% and 95% amino acid (aa) sequences identity with mouse and rat Gelsolin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GSN |
Protein | Gelsolin |
Uniprot ID | P06396 |
Function | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. |
Tissue Specificity | Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. |
Sub-cellular localization | Isoform 2: Cytoplasm, cytoskeleton. |
Sequence Similarities | Belongs to the villin/gelsolin family. |
Aliases | Actin depolymerizing factor antibody|Actin-depolymerizing factor antibody|ADF antibody|AGEL antibody|Brevin antibody|DKFZp313L0718 antibody|GELS_HUMAN antibody|Gelsolin antibody|GSN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Gelsolin antibody, ASA-B0780, Western blotting
All lanes: Anti Gelsolin (ASA-B0780) at 0.5ug/ml
WB: Recombinant Human Gelsolin Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti Gelsolin (ASA-B0780) at 0.5ug/ml
WB: Recombinant Human Gelsolin Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- Gelsolin antibody, ASA-B0780, Western blotting
All lanes: Anti Gelsolin (ASA-B0780) at 0.5ug/ml
WB: A431 Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 86KD
All lanes: Anti Gelsolin (ASA-B0780) at 0.5ug/ml
WB: A431 Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 86KD
Anti- Gelsolin antibody, ASA-B0780, IHC(P)
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue