More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glial cell derived neurotrophic factor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human GDNF(142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GDNF |
Protein | Glial cell line-derived neurotrophic factor(hGDNF) |
Uniprot ID | P39905 |
Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. |
Tissue Specificity | In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the TGF-beta family. GDNF subfamily. |
Aliases | gdnf antibody|GDNF_HUMAN antibody|Glial cell derived neurotrophic factor antibody|Glial Cell Line Derived Neurotrophic Factor antibody|Glial cell line-derived neurotrophic factor antibody|Glial derived neurotrophic factor antibody|HFB1 GDNF antibody|hGDNF antibody|HSCR3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GDNF antibody, ASA-B0776, Western blotting
Lane 1: Recombinant Human GDNF Protein 10ng
Lane 2: Recombinant Human GDNF Protein 5ng
Lane 3: Recombinant Human GDNF Protein 2.5ng
Lane 4: SMMC Cell Lysate
Lane 1: Recombinant Human GDNF Protein 10ng
Lane 2: Recombinant Human GDNF Protein 5ng
Lane 3: Recombinant Human GDNF Protein 2.5ng
Lane 4: SMMC Cell Lysate
Anti-GDNF antibody, ASA-B0776, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-GDNF antibody, ASA-B0776, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue