More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| growth associated protein 43 | Monoclonal | IgG | Mouse | Human, Mouse, Rat | IHC-P, IHC-F, WB | Ascites |
Immunogen |
| GAP-43 from neonatal rat forebrain membranes. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GAP43 |
Protein | Neuromodulin |
Uniprot ID | Human(P17677), Mouse(P06837), Rat(P07936) |
Function | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the neuromodulin family. |
Aliases | B-50|PP46|protein F1|axonal membrane protein GAP-43|calmodulin-binding protein P-57|nerve growth-related peptide GAP43|neural phosphoprotein B-50| neuron growth-associated protein 43 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-1μg/ml | Human, mouse, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 1-2μg/ml | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GAP43 antibody, ASA-B0763, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
-ASA-B0763-1.jpg)
-ASA-B0763-2.jpg)