More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| junction plakoglobin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human gamma Catenin(8-22aa EQPIKVTEWQQTYTY), identical to the related mouse and rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | JUP |
Protein | Junction plakoglobin |
Uniprot ID | P14923 |
Function | Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cell junction, adherens junction. |
Sequence Similarities | Belongs to the beta-catenin family. |
Aliases | ARVD 12 antibody|ARVD12 antibody|Catenin(cadherin associated protein) gamma 80kDa antibody|Catenin(cadherin associated protein), gamma 80kDa antibody|catenin(cadherin-associated protein) gamma(80kD) antibody|Catenin gamma 80kDa antibody|Catenin gamma antibody|CTNNG antibody|Desmoplakin 3 antibody|Desmoplakin III antibody|Desmoplakin-3 antibody|Desmoplakin3 antibody|DesmoplakinIII antibody|DP 3 antibody|DP III antibody|DP3 antibody|DPIII antibody|Gamma catenin antibody|Junction plakoglobin antibody|JUP antibody|OTTHUMP00000164732 antibody|OTTHUMP00000164735 antibody|OTTHUMP00000164738 antibody|PDGB antibody|PKGB antibody|PLAK_HUMAN antibody|PLAKOGLOBIN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-gamma Catenin antibody, ASA-B0760, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Anti-gamma Catenin antibody, ASA-B0760, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
