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gamma Catenin Antibody

ASA-B0761

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
junction plakoglobin Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, IHC-F, ICC, WB Immunogen affinity purified.

Immunogen

E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745). Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 JUP

Protein

 Junction plakoglobin

Uniprot ID

 P14923

Function

 Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

Tissue Specificity

Sub-cellular localization

 Cell junction, adherens junction.

Sequence Similarities

 Belongs to the beta-catenin family.

Aliases

 ARVD 12 antibody|ARVD12 antibody|Catenin (cadherin associated protein) gamma 80kDa antibody|Catenin (cadherin associated protein), gamma 80kDa antibody|catenin (cadherin-associated protein) gamma (80kD) antibody|Catenin gamma 80kDa antibody|Catenin gamma antibody|CTNNG antibody|Desmoplakin 3 antibody|Desmoplakin III antibody|Desmoplakin-3 antibody|Desmoplakin3 antibody|DesmoplakinIII antibody|DP 3 antibody|DP III antibody|DP3 antibody|DPIII antibody|Gamma catenin antibody|Junction plakoglobin antibody|JUP antibody|OTTHUMP00000164732 antibody|OTTHUMP00000164735 antibody|OTTHUMP00000164738 antibody|PDGB antibody|PKGB antibody|PLAK_HUMAN antibody|PLAKOGLOBIN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Rat HumanAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- gamma Catenin antibody, ASA-B0761, Western blotting
All lanes: Anti gamma Catenin(ASA-B0761) at 0.5ug/ml
WB: Recombinant Human gamma Catenin Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti- gamma Catenin antibody, ASA-B0761, Western blotting
All lanes: Anti gamma Catenin (ASA-B0761) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Rat Thymus Tissue Lysate at 50ug
Lane 4: RH35 Whole Cell Lysate at 40ug
Lane 5: Hela Whole Cell Lysate at 40ug
Lane 6: Colo320 Whole Cell Lysate at 40ug
Lane 7: HepG2 Whole Cell Lysate at 40ug
Lane 8: HepA Whole Cell Lysate at 40ug
Lane 9: MC
Anti- gamma Catenin antibody, ASA-B0761, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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