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Galectin 3 Antibody

ASA-B0756

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
lectin, galactoside-binding, soluble, 3 Polyclonal IgG Rabbit Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived mouse Galectin 3 recombinant protein (Position: K153-I264). Mouse Galectin 3 shares 88% and 91% amino acid (aa) sequence identity with human and rat Galectin 3, respectively.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 LGALS3

Protein

 Galectin-3

Uniprot ID

 P16110

Function

 Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells (By similarity).

Tissue Specificity

The highest levels are found in activated macrophages.

Sub-cellular localization

 Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface.

Sequence Similarities

Aliases

 35 kDa lectin antibody|Carbohydrate binding protein 35 antibody|Carbohydrate-binding protein 35 antibody|CBP 35 antibody|CBP35 antibody|GAL 3 antibody|Gal-3 antibody|GAL3 antibody|Galactose specific lectin 3 antibody|Galactose-specific lectin 3 antibody|Galactoside binding protein antibody|Galactoside-binding protein antibody|GALBP antibody|Galectin 3 internal gene,included antibody|Galectin-3 antibody|Galectin3 antibody|GALIG antibody|GBP antibody|IgE binding protein antibody|IgE-binding protein antibody|L 31 antibody|L 34 antibody|L-31 antibody|L-34 galactoside-binding lectin antibody|L31 antibody|Laminin binding protein antibody|Laminin-binding protein antibody|Lectin galactose binding soluble 3 antibody|Lectin L 29 antibody|Lectin L-29 antibody|Lectin, galactose binding, soluble 3 antibody|LEG3_HUMAN antibody|LGALS 3 antibody|LGALS2 antibody|LGALS3 antibody|LGALS3 protein antibody|MAC 2 antigen antibody|Mac-2 antibody|Mac-2 antigen antibody|MAC2 antibody|Macrophage galactose-specific lectin antibody|MGC105387 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Galectin3 antibody, ASA-B0756, Western blotting
All lanes: Anti Galectin3 (ASA-B0756) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: Mouse Ovary Tissue Lysate at 50ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: ANA-1 Whole Cell Lysate at 40ug
Lane 7: NIH3T3 Whole Cell Lysate at 40ug
redicted bind size: 26KD
Observed bind size: 26KD
Anti- Galectin3 antibody, ASA-B0756, IHC(P)
IHC(P): Mouse Spleen Tissue
Anti- Galectin3 antibody, ASA-B0756, IHC(P)
IHC(P): Rat Intestine Tissue

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