More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lectin, galactoside-binding, soluble, 3 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LGALS3 |
Protein | Galectin-3 |
Uniprot ID | P17931 |
Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. |
Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. |
Sub-cellular localization | Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface. |
Sequence Similarities | Contains 1 galectin domain. |
Aliases | 35 kDa lectin antibody|Carbohydrate binding protein 35 antibody|Carbohydrate-binding protein 35 antibody|CBP 35 antibody|CBP35 antibody|GAL 3 antibody|Gal-3 antibody|GAL3 antibody|Galactose specific lectin 3 antibody|Galactose-specific lectin 3 antibody|Galactoside binding protein antibody|Galactoside-binding protein antibody|GALBP antibody|Galectin 3 internal gene,included antibody|Galectin-3 antibody|Galectin3 antibody|GALIG antibody|GBP antibody|IgE binding protein antibody|IgE-binding protein antibody|L 31 antibody|L 34 antibody|L-31 antibody|L-34 galactoside-binding lectin antibody|L31 antibody|Laminin binding protein antibody|Laminin-binding protein antibody|Lectin galactose binding soluble 3 antibody|Lectin galactoside binding soluble 3 antibody|Lectin L 29 antibody|Lectin L-29 antibody|Lectin, galactose binding, soluble 3 antibody|LEG3_HUMAN antibody|LGALS 3 antibody|LGALS2 antibody|LGALS3 antibody|LGALS3 protein antibody|MAC 2 antigen antibody|Mac-2 antibody|Mac-2 antigen antibody|MAC2 antibody|Macrophage galactose-specific lectin antibody|MGC105387 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Galectin 3 antibody, ASA-B0755--1.jpg
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-Galectin 3 antibody, ASA-B0755--2.jpg
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-Galectin 3 antibody, ASA-B0755--3.jpg
All lanes: Anti-Galectin-3(ASA-B0755) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: Smmc Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
All lanes: Anti-Galectin-3(ASA-B0755) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: Smmc Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD