More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lectin, galactoside-binding, soluble, 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Galectin 1 recombinant protein (Position: A2-D135). Human Galectin 1 shares 88% and 90% amino acid (aa) sequences identity with mouse and rat Galectin 1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LGALS1 |
Protein | Galectin-1 |
Uniprot ID | P09382 |
Function | May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. |
Tissue Specificity | Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Contains 1 galectin domain. |
Aliases | 14 kDa laminin-binding protein antibody|14 kDa lectin antibody|Beta galactoside binding lectin antibody|Beta galactoside binding lectin L 14 I antibody|beta galactoside binding protein antibody|Beta-galactoside-binding lectin L-14-I antibody|DKFZp686E23103 antibody|Gal 1 antibody|Gal-1 antibody|GAL1 antibody|Galaptin antibody|Galbp antibody|Galectin antibody|Galectin-1 antibody|Galectin1 antibody|GBP antibody|HBL antibody|HLBP14 antibody|HPL antibody|L 14.5 antibody|L-14.5 antibody|L14 antibody|Lactose binding lectin 1 antibody|Lactose-binding lectin 1 antibody|Lect14 antibody|Lectin galactoside binding soluble 1 antibody|Lectin galactoside-binding soluble 1 antibody|LEG1_HUMAN antibody|LGALS 1 antibody|LGALS1 antibody|Lgals1 lectin galactose binding soluble 1 antibody|MAPK activating protein MP12 antibody|Putative MAPK activating protein MP12 antibody|Putative MAPK-activating protein PM12 antibody|S Lac lectin 1 antibody|S-Lac lectin 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Galectin1 antibody, ASA-B0754, Western blotting
All lanes: Anti Galectin1(ASA-B0754) at 0.5ug/ml
WB: Recombinant Human Galectin1 Protein 0.5ng
Predicted bind size: 16KD
Observed bind size: 16KD
All lanes: Anti Galectin1(ASA-B0754) at 0.5ug/ml
WB: Recombinant Human Galectin1 Protein 0.5ng
Predicted bind size: 16KD
Observed bind size: 16KD
Anti- Galectin1 antibody, ASA-B0754, Western blotting
All lanes: Anti Galectin1(ASA-B0754) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: NRK Whole Cell Lysate at 40ug
Predicted bind size: 15KD
Observed bind size: 15KD
All lanes: Anti Galectin1(ASA-B0754) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: NRK Whole Cell Lysate at 40ug
Predicted bind size: 15KD
Observed bind size: 15KD
Anti- Galectin1 antibody, ASA-B0754,IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue