More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 6(neurotransmitter transporter, GABA), member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human GABA Transporter 1(473-487aa WFYGVNRFYDNIQEM), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC6A1 |
Protein | Sodium- and chloride-dependent GABA transporter 1(GAT-1) |
Uniprot ID | P30531 |
Function | Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Note: Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). |
Sequence Similarities | |
Aliases | GABATHG antibody|GABATR antibody|GABT 1 antibody|GABT1 antibody|GAT-1 antibody|GAT1 antibody|SC6A1_HUMAN antibody|SLC6A1 antibody|Sodium and chloride dependent GABA transporter 1 antibody|Sodium- and chloride-dependent GABA transporter 1 antibody|Solute carrier family 6(neurotransmitter transporter GABA) member 1 antibody|Solute carrier family 6 member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GABA Transporter 1/GAT 1 antibody, ASA-B0747, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Anti-GABA Transporter 1/GAT 1 antibody, ASA-B0747, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
