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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| frizzled class receptor 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Frizzled homolog 1 (369-400aa YIAGFLLEDRVVCNDKFAEDGARTVAQG TKKE), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FZD1 |
Protein | Frizzled-1 |
Uniprot ID | Q9UP38 |
Function | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. |
Tissue Specificity | Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . |
Sequence Similarities | Belongs to the G-protein coupled receptor Fz/Smo family. |
Aliases | Chfz-1 antibody|Dfrizzled-1 antibody|DKFZp564G072 antibody|FLJ95923 antibody|Frizzled 1 antibody|Frizzled homolog 1 (Drosophila) antibody| Frizzled homolog 1 antibody|Frizzled-1 antibody|Fz receptor 1 antibody|Fz-1 antibody|Fz1 antibody|FZD1 antibody|FZD1_HUMAN antibody|FzE1 antibody|hFz1 antibody|Rfz1 antibody|Wnt receptor antibody|Xfrizzled-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.6μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-2μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Frizzled homolog 1 antibody, ASA-B0735, Western blotting
All lanes: Anti Frizzled homolog 1 (ASA-B0735) at 0.5ug/ml
Lane 1: 22RV1 Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71 KD
All lanes: Anti Frizzled homolog 1 (ASA-B0735) at 0.5ug/ml
Lane 1: 22RV1 Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71 KD
Anti- Frizzled homolog 1 antibody, ASA-B0735, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
