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FOXO3A Antibody View larger

FOXO3A Antibody

ASA-B0730

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
forkhead box O3 Polyclonal IgG Rabbit Human, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673). Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 FOXO3

Protein

 Forkhead box protein O3

Uniprot ID

 O43524

Function

 Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Tissue Specificity

Ubiquitous.

Sub-cellular localization

 Cytoplasm, cytosol. Nucleus. Note: Translocates to the nucleus upon oxidative stress and in the absence of survival factors.

Sequence Similarities

 Contains 1 fork-head DNA-binding domain.

Aliases

 AF6q21 antibody|AF6q21 protein antibody|DKFZp781A0677 antibody|FKHR2 antibody|FKHRL 1 antibody|FKHRL1 antibody|FKHRL1P2 antibody|Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody|Forkhead box O3 antibody|Forkhead box O3A antibody|Forkhead box protein O3 antibody|Forkhead box protein O3A antibody|Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody|Forkhead homolog (rhabdomyosarcoma) like 1 antibody|Forkhead in rhabdomyosarcoma like 1 antibody|Forkhead in rhabdomyosarcoma-like 1 antibody|FOX O3A antibody|FOXO2 antibody|foxo3 antibody|FOXO3_HUMAN antibody|FOXO3A antibody|MGC12739 antibody|MGC31925 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FOXO3A antibody, ASA-B0730, Western blotting
All lanes: Anti FOXO3A (ASA-B0730) at 0.5ug/ml
WB: Recombinant Human FOXO3A Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- FOXO3A antibody, ASA-B0730, Western blotting
All lanes: Anti FOXO3A (ASA-B0730) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: PC-12Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: K562 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
Anti- FOXO3A antibody, ASA-B0730,IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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