More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| forkhead box O3 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673). Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FOXO3 |
Protein | Forkhead box protein O3 |
Uniprot ID | O43524 |
Function | Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Cytoplasm, cytosol. Nucleus. Note: Translocates to the nucleus upon oxidative stress and in the absence of survival factors. |
Sequence Similarities | Contains 1 fork-head DNA-binding domain. |
Aliases | AF6q21 antibody|AF6q21 protein antibody|DKFZp781A0677 antibody|FKHR2 antibody|FKHRL 1 antibody|FKHRL1 antibody|FKHRL1P2 antibody|Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody|Forkhead box O3 antibody|Forkhead box O3A antibody|Forkhead box protein O3 antibody|Forkhead box protein O3A antibody|Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody|Forkhead homolog (rhabdomyosarcoma) like 1 antibody|Forkhead in rhabdomyosarcoma like 1 antibody|Forkhead in rhabdomyosarcoma-like 1 antibody|FOX O3A antibody|FOXO2 antibody|foxo3 antibody|FOXO3_HUMAN antibody|FOXO3A antibody|MGC12739 antibody|MGC31925 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FOXO3A antibody, ASA-B0730, Western blotting
All lanes: Anti FOXO3A (ASA-B0730) at 0.5ug/ml
WB: Recombinant Human FOXO3A Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti FOXO3A (ASA-B0730) at 0.5ug/ml
WB: Recombinant Human FOXO3A Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- FOXO3A antibody, ASA-B0730, Western blotting
All lanes: Anti FOXO3A (ASA-B0730) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: PC-12Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: K562 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
All lanes: Anti FOXO3A (ASA-B0730) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: PC-12Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: K562 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
Anti- FOXO3A antibody, ASA-B0730,IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue