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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| FBJ murine osteosarcoma viral oncogene homolog B | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Fos B (264-291aa LPFQTSQDAPPNLTASLFTHSEVQVLGD), different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FOSB |
Protein | Protein fosB |
Uniprot ID | P53539 |
Function | FosB interacts with Jun proteins enhancing their DNA binding activity. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the bZIP family. Fos subfamily. |
Aliases | DKFZp686C0818 antibody|FBJ murine osteosarcoma viral oncogene homolog B antibody|fosB antibody|FOSB_HUMAN antibody|G0/G1 switch regulatory protein 3 antibody|G0S3 antibody|GOS 3 antibody|GOS B antibody|GOS3 antibody|GOSB antibody|MGC42291 antibody|Oncogene FOS B antibody| Oncogene FOSB antibody|Protein fos B antibody|Protein fosB antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Fos B antibody, ASA-B0727, Western blotting
All lanes: Anti Fos B (ASA-B0727) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti Fos B (ASA-B0727) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- Fos B antibody, ASA-B0727, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- Fos B antibody, ASA-B0727, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue