More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Friend leukemia virus integration 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FLI1 |
Protein | Friend leukemia integration 1 transcription factor |
Uniprot ID | Q01543 |
Function | Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the ETS family. |
Aliases | ERGB transcription factor antibody|Ewing Sarcoma breakpoint region 2 antibody|EWSR 2 antibody|EWSR2 antibody|FLI 1 antibody|FLI 1 proto oncogene antibody|FLI1 antibody|FLI1 EWS fusion gene antibody|FLI1 proto oncogene antibody|FLI1_HUMAN antibody|Friend leukemia integration 1 transcription factor antibody|Friend leukemia virus integration 1 antibody|Proto-oncogene Fli-1 antibody|SIC 1 antibody|SIC1 antibody|Transcription factor ERGB antibody|Viral integration region FLI1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FLI1 antibody, ASA-B0717, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-FLI1 antibody, ASA-B0717, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
Anti-FLI1 antibody, ASA-B0717, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue