FLI1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
Friend leukemia virus integration 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	FLI1
Protein	Friend leukemia integration 1 transcription factor
Uniprot ID	Q01543
Function	Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Tissue Specificity
Sub-cellular localization	Nucleus.
Sequence Similarities	Belongs to the ETS family.
Aliases	ERGB transcription factor antibody|Ewing Sarcoma breakpoint region 2 antibody|EWSR 2 antibody|EWSR2 antibody|FLI 1 antibody|FLI 1 proto oncogene antibody|FLI1 antibody|FLI1 EWS fusion gene antibody|FLI1 proto oncogene antibody|FLI1_HUMAN antibody|Friend leukemia integration 1 transcription factor antibody|Friend leukemia virus integration 1 antibody|Proto-oncogene Fli-1 antibody|SIC 1 antibody|SIC1 antibody|Transcription factor ERGB antibody|Viral integration region FLI1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information