More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| flavin containing monooxygenase 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Flavin containing monooxygenase 4(75-92aa HEDYPNFMNHEKFWDYLQ), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FMO4 |
Protein | Dimethylaniline monooxygenase [N-oxide-forming] 4 |
Uniprot ID | P31512 |
Function | This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. |
Tissue Specificity | Liver. |
Sub-cellular localization | Microsome membrane. Endoplasmic reticulum membrane. |
Sequence Similarities | Belongs to the FMO family. |
Aliases | Dimethylaniline monooxygenase[N oxide forming] 4 antibody|Dimethylaniline monooxygenase[N-oxide-forming] 4 antibody|Dimethylaniline monooxygenase 4 antibody|Dimethylaniline oxidase 4 antibody|FMO 2 antibody|FMO 4 antibody|FMO2 antibody|Fmo4 antibody|FMO4_HUMAN antibody|Hepatic flavin containing monooxygenase 4 antibody|Hepatic flavin-containing monooxygenase 4 antibody|OTTHUMP00000060454 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Flavin containing monooxygenase 4 antibody, ASA-B0716, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 5: A431 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 5: A431 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Anti-Flavin containing monooxygenase 4 antibody, ASA-B0716, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue
