More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| fibronectin 1 | Monoclonal | IgG | Mouse | Chicken, Human, Mouse | IHC-P, IHC-F, ICC, WB | Ascites |
Immunogen |
| Fibronectin from human plasma. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FN1 |
Protein | Fibronectin |
Uniprot ID | Human(P02751), Mouse(P11276), Rat(P04937) |
Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. |
Tissue Specificity | Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Contains 12 fibronectin type-I domains. |
Aliases | FN|CIG|FNZ|MSF|ED-B|FINC|GFND|LETS|GFND2 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 1-2μg/ml | Human, chicken, mouse. | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, chicken, mouse. | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 1-2μg/ml | Human, chicken, mouse. | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human, chicken, mouse. | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
