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FHIT Antibody

ASA-B0711

$365.00

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Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
fragile histidine triad Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequences identity with mouse and rat FHIT, respectively.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 FHIT

Protein

 Bis(5'-adenosyl)-triphosphatase

Uniprot ID

 P49789

Function

 Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

Tissue Specificity

Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.

Sub-cellular localization

 Cytoplasm. Mitochondrion. Nucleus.

Sequence Similarities

 Contains 1 HIT domain.

Aliases

 AP3A hydrolase antibody|AP3A hydrolase fragile site 3p14.2 antibody|AP3Aase antibody|Bis 5' adenosyl triphosphatase antibody|Bis(5' adenosyl) triphosphatase antibody|Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody|Dinucleosidetriphosphatase antibody|FRA 3B antibody|FRA3B antibody|Fragile histidine triad antibody|Fragile histidine triad gene antibody|Fragile histidine triad protein antibody|Tumor suppressor protein antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FHIT antibody, ASA-B0711, Western blotting
All lanes: Anti FHIT (ASA-B0711) at 0.5ug/ml
WB: Recombinant Human FHIT Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- FHIT antibody, ASA-B0711, Western blotting
All lanes: Anti FHIT (ASA-B0711) at 0.5ug/ml
Lane 1: HT1080 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Predicted bind size: 17KD
Observed bind size: 17KD
Anti- FHIT antibody, ASA-B0711, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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