FGFR1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
fibroblast growth factor receptor 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FGFR1(806-822aa CLPRHPAQLANCGLKRR), different from the related mouse sequence by two amino acids and rat sequence by three amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	FGFR1
Protein	Fibroblast growth factor receptor 1(FGFR-1/bFGF-R-1)
Uniprot ID	P11362
Function	Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Tissue Specificity	Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
Sub-cellular localization	Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. Note: After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus.
Sequence Similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Aliases	Basic fibroblast growth factor receptor 1 antibody|bFGF R antibody|bFGF-R-1 antibody|BFGFR antibody|C FGR antibody|CD 331 antibody|CD331 antibody|CD331 antigen antibody|CEK antibody|FGFBR antibody|FGFR 1 antibody|FGFR-1 antibody|FGFR1 antibody|FGFR1_HUMAN antibody|Fibroblast growth factor receptor 1 antibody|FLG antibody|FLG protein antibody|FLJ14326 antibody|FLT 2 antibody|FLT-2 antibody|FLT2 antibody|FMS like tyrosine kinase 2 antibody|Fms like tyrosine kinase 2 antibody|Fms related tyrosine kinase 2 antibody|Fms related tyrosine kinase 2 Pfeiffer syndrome antibody|Fms-like tyrosine kinase 2 antibody|H2 antibody|H3 antibody|H4 antibody|H5 antibody|HBGFR antibody|Heparin binding growth factor receptor antibody|Hydroxyaryl protein kinase antibody|KAL 2 antibody|KAL2 antibody|MFR antibody|N SAM antibody|N sam tyrosine kinase antibody|Protein tyrosine kinase antibody|Proto-oncogene c-Fgr antibody|Tyrosylprotein kinase antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information