More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| fibroblast growth factor 9(glia-activating factor) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human FGF9(150-164aa SNLYKHVDTGRRYYV), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FGF9 |
Protein | Fibroblast growth factor 9(FGF-9) |
Uniprot ID | P31371 |
Function | Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. |
Tissue Specificity | Glial cells. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the heparin-binding growth factors family. |
Aliases | FGF 9 antibody|FGF-9 antibody|FGF9 antibody|FGF9_HUMAN antibody|Fibroblast Growth Factor 9 antibody|GAF antibody|Glia Activating Factor antibody|Glia-activating factor antibody|HBFG 9 antibody|HBFG9 antibody|HBGF-9 antibody|Heparin-binding growth factor 9 antibody|MGC119914 antibody|MGC119915 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FGF9 antibody, ASA-B0705, Western blotting
All lanes: Anti FGF9 (ASA-B0705) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti FGF9 (ASA-B0705) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Anti-FGF9 antibody, ASA-B0705, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
