More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
F-box and leucine-rich repeat protein 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human FbxL4(552-565aa DELASNCTRLQQLD), different from the related mouse and rat sequences by one amino acid. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FBXL4 |
Protein | F-box/LRR-repeat protein 4 |
Uniprot ID | Q9UKA2 |
Function | |
Tissue Specificity | Expressed in heart, kidney, liver, lung, pancreas, and placenta, but not in skeletal muscle. |
Sub-cellular localization | Cytoplasm. Nucleus. Mitochondrion. |
Sequence Similarities | Contains 1 F-box domain. |
Aliases | F box and leucine rich repeat protein 4 antibody|F box protein FBL4/FBL5 antibody|F box/LRR repeat protein 4 antibody|F-box and leucine-rich repeat protein 4 antibody|F-box protein FBL 4 antibody|F-box protein FBL4/FBL5 antibody|F-box/LRR-repeat protein 4 antibody|FBL 4 antibody|FBL 5 antibody|FBL4 antibody|FBL5 antibody|Fbxl4 antibody|FBXL4_HUMAN antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FbxL4 antibody, ASA-B0692, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: PANC Cell Lysate
Anti-FbxL4 antibody, ASA-B0692, IHC(P)
IHC(P): Human Lung Cancer Tissue