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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Fas ligand(TNF superfamily, member 6) | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(192-218aa YSKVYFRGQSCNNLPLSHKVYMRNSKY), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FASLG |
Protein | Tumor necrosis factor ligand superfamily member 6 |
Uniprot ID | P48023 |
Function | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Note: Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane. |
Sequence Similarities | Belongs to the tumor necrosis factor family. |
Aliases | ALPS1B antibody|APO1L antibody|Apoptosis(APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|FAS antigen ligand antibody|Fas L antibody|Fas ligand(TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand(TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNF superfamily member 6 antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor(ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Fas Ligand antibody, ASA-B0685, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HT1080 Cell Lysate
Lane 6: COLO320 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HT1080 Cell Lysate
Lane 6: COLO320 Cell Lysate
Anti-Fas Ligand antibody, ASA-B0685, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
